ISOLASI DNA DAN AMPLIFIKASI GEN COX3 PADA IKAN SELAIS KAPOREH (Ompok eugeneiatus Vaillant 1893) DARI SUNGAI KAMPAR, PROVINSI RIAU
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Date
2016-01-26
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Abstract
Selais Kaporeh (Ompok eugeneiatus) is an endemic fish in floodplain river. The genetic information of Ompok eugeneiatus is still limited. This research aims to isolate and amplify Cytochrome c Oxydase sub unit 3 (COX3) gene from Ompok eugeneiatus originated from Kampar River. This study was conducted from November 2014 to April 2015. Cytochrome c Oxydase sub unit 3 gene was amplified using COX3-F1 and COX3-R1 primers which were design from COX3 gene of Ictalurus punctatus (GenBank 2004). PCR condition based on Elvyra and Duryadi (2007) with modification. The result indicated that COX3-F1 and COX3-R1 primers can be used to amplify COX3 gene of Ompok eugeneiatus with size of 642 bp.
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Amplify, gen COX3, isolate, Ompok eugeneiatus