ISOLASI METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK n-HEKSANA DARI DAUN BAKAU Rhizopora mucronata (Rhizophoraceae)
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Date
2020-10
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Abstract
Rhizophora mucronata is type of mangrove that has diverse secondary
metabolites, such us alkaloids, flavonoids, tannins and phenol compounds. The
purpose of this study was to isolate secondary metabolites and to determine
toxicity test by BSLT method. Leave extraction was carried out using ethanol and
then fractionated with n-hexane. The fractionation produced 40 g dark green.
Crude n-hexane extract was separated using liquid vacuum chromatography and 6
fractions produced. White crystals were isolated from second fraction of n-hexane
with melting poin of 236-238 oC. The crystals were characterized using UV and
FT-IR spectroscopy. UV spectrum of RmHF2 indicated absorption at λmax 292
nm. The FT-IR absorption spectrum showed absorption peaks at 1031 (C-O),1376
(C-H) methyl, 1463 (C-H) methylen,1605 (C=C), 1697 (C=O) dan 2942 cm-1 (CH
aliphatic). The result of toxicity test by BSLT (Brine Shrimps Lethality Test)
method on that crude extract was non-toxic with LC50 value of 841395 ppm.
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Keywords
isolation, rhizophora mucronata, toxicity