ISOLASI DAN UJI TOKSISITAS SENYAWA METABOLIT SEKUNDER DARI EKSTRAK n-HEKSANA TUMBUHAN PAKU AHAKA (Vittaria elongata Sw)
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Date
2020-10
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Abstract
elongata Sw ferns is one of the fern plants known as ahaka fern area wich is widely
distributed in kampar and pelalawan, Riau Province. The study was to isolate and
then characterize the pure compounds obtained from extract n-hexana and to
determine the toxicity of the secondary metabolites compound by the Brine Shrimp
Lethality Test (BSLT) method. Extraction techniques were applied by using
maceration method with methanol, continued fractionation by partition using nhexane.
Secondary metabolites compounds were separated by using liquid
chromatography and flash chromatography, then the pure compounds were
characterized by FTIR. A compound obtained the result of isolation in the form of the
brownish white amorphous solid with melting point of 219-221˚C. Of toxicity test
that is good on n-hexane extract with the value of the LC₅ₒ was showed 161,163
μg/mL, from the data above was concluded that compound was toxic.
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Keywords
Extraction, Partition, V.elongata Sw