ISOLASI MINYAK ATSIRI DAUN KEMANGI (Ocimum sanctum L) CARA KONVENSIONAL DAN MICROWAVE SERTA UJI AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN
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Date
2016-10-19
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Abstract
The aim of this study was to isolate essential oils from Ocimum sanctum L leaves by
Clevenger-hydrodistillation and microwave-assisted hydrodistillation. Components of
the essential oil were then identified using Gas Chromatography Mass Spectrometry
(GC-MS). Antimicrobial activity of these oils was determined by this diffusion method
against Escherichia coli, the main component of the essential oils was geranial
(24.74%) and neral (28.11%) for Clevenger-hydrodistillation method, while geranial
(41.85%) and neral (29.07%) for the method microwave-assisted hydrodistillation. Our
finding for antimicrobial test showed that essential oil of microwave-assisted
hydrodistillation was more active than those of Clevenger-hydrodistillation. Antioxidant
activity using DPPH assay of the essential oil of Clevenger-hydrodistillation was more
active (IC50=513.1802 μg/mL) than those of microwave-assisted hydrodistillation
(IC50=713.2279 μg/mL).
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Keywords
Ocimum sanctum L, Essential oil, GC-MS, Antibacterial, Antioxidant