Optimalisasi produksi enzim selulase isolat selulolitik yang diisolasi dari Sungai Siak
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Date
2013-04-16
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Abstract
Isolates of S-16 and S-22 are some of the local strains producing cellulases that have been
isolated from the Siak River water. Cellulase enzyme can be degrades of cellulosewaste
containing that enable for the processing of waste to economically priced products such as
glucose. In this research, optimization of the production of cellulase enzymes from isolates of
S-16 and S-22 with a variety of media composition, temperature and concentration of carbon
source production. The quantitative assay for enzyme activity was determined by Nelson
Somogyi method with carboxymethyl of cellulose (CMC) as a substrate. The highest
cellulase enzyme activity of S-16 obtained in a media production (KH2P04; MgS04.7H20;
NaN03; CaC12; NaCl; CMC) of 88x10-3 U / mL, and the optimum concentration of source
of carbon at 1% obtained for 50.36 xlO-3 U / mL, whereas of S-22 obtained in a media
production (KH2P04; MgS04.7H20; FeS04.7H20; NaN03; KCI; CMC) of 14x10-3 U /
mL, and the optimum concentration of sources of carbon at 1% obtained for 14 , 7x10-3 U /
mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test
multiple range at level 5%.
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Keywords
Cellulosa, Cellulase, Carboxymethil Cellulosa