OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH atpB-rbcL INTERGENIC SPACER PADA TUMBUHAN DURIK-DURIK (Syzygiumsp.) ASAL RIAU

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dc.contributor.authorPutri, Nur Aisyah
dc.contributor.supervisorHerman, Herman
dc.date.accessioned2021-07-28T04:56:30Z
dc.date.available2021-07-28T04:56:30Z
dc.date.issued2020-08
dc.description.abstractDurik-durik (Syzygium sp.) is a plant from the family Myrtaceae that can grow in the flooded swamp ecosystem. This plant has a good adaptability to inundation stress. This study aimed to determine the optimal annealing temperature that could be used for amplification in the atpB-rbcL intergenic spacer region. Leaf samples of durik-durik (Syzygium sp.) plant were taken from Lake Kajuik located in Langgam Village, Pelalawan Regency, Riau Province. Samples were extracted using Genomic DNA mini kit Plant (Geneaid) then amplified using dreamTaq DNA polymerase and atpB-rbcL intergenic spacer primer pair with a PCR machine. The results showed that DNA bands were around 1000 bp. The optimal annealing temperature was Tm-5 = 46,6 oC.en_US
dc.description.sponsorshipDosen Bidang Genetika Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riauen_US
dc.identifier.otherwahyu sari yeni
dc.identifier.urihttps://repository.unri.ac.id/handle/123456789/10065
dc.language.isoenen_US
dc.subjectatpB-rbcL intergenic spacer, durik-durik (Syzygium sp.)en_US
dc.subjectKajuik Lakeen_US
dc.subjectPCRen_US
dc.subjecttemperature optimizationen_US
dc.titleOPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI DAERAH atpB-rbcL INTERGENIC SPACER PADA TUMBUHAN DURIK-DURIK (Syzygiumsp.) ASAL RIAUen_US
dc.typeArticleen_US

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