ISOLASI DAN UJI TOKSISITAS EKSTRAK ETIL ASETAT DARI BENALU JENGKOL Scurrula ferruginea
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Date
2023-09
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Elfitra
Abstract
Scurrula ferruginea belongs to the Loranthaceae family which lives on its host, the
jengkol tree. Ethyl acetate is a solvent with low toxicity that is semipolar so it is
expected to attract compounds that are both polar and non-polar. This research aims
to isolate secondary metabolites and test the toxicity of the jengkol parasite plant
from ethyl acetate extract using the brine shrimp lethality test (BSLT) method. The
samples were extracted using the maceration method using methanol solvent and
partitioned using n-hexane and ethyl acetate solvents. The ethyl acetate fraction was
separated using vacuum liquid chromatography (VLC) which produced 7 fractions.
Fractions 2 and 3 were separated using gravity column chromatography which
produced 6 fractions. In fraction 5, the ethyl acetate extract produced a pure
compound in the form of a white powder with a melting point of 279-281°C and
codenamed SFEA-F5. The FTIR spectrum was obtained at wavenumber of 3356
cm-1 (OH),
2933 cm-1 ( aliphatic C-H), 1455 cm-1 (CH2), 1021-1166 cm-1 (C-O).
Based on the results of the characterization of the SFEA-F5 compound, it is a class
of terpenoid compounds. The results of the toxicity test with BSLT showed that the
ethyl acetate extract was toxic with an LC50 value of 115,63 ppm.
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Keywords
isolation, scurrula ferruginea, toxicity
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