MOTILITAS SPERMATOZOA SAPI BALI DIKRIOPRESERVASI DALAM PENGENCER TRIS-KUNING TELUR DENGAN EKSTRAK DAUN KELOR (Moringa oleifera)
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Date
2023-05
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Elfitra
Abstract
Bali cattle is one of the potential germplasm to be developed, one of which is by
cryopreservation process. This study aimed to determine the effectiveness of giving
moringa leaf extract (Moringa oleifera) into tris-egg yolk diluent on the motility of
spermatozoa in bali cattle with various of freezing time (0, 2, and 8 days). Examination
of spermatozoa motility was carried out using the Computerized Assisted Sperm
Analyzed (CASA) system. The stages of the research were extracting moringa leaves,
making diluent, giving moringa leaf extract to tris-yolk diluent, storing semen,
examining fresh semen, diluting and preparing frozen semen, as well as examining
spermatozoa motility after cryopreservation. Data were analyzed using the Analysis of
Variance (ANOVA) test and Duncan's test using SPSS 17.0 program. Result of study
showed that the addition of moringa leaf extract to diluent had a significant effect
(p<0.05) on spermatozoa motility of bali cattle without freezing treatment. Additional of
0.03 g moringa leaf extract showed the best results on motility values compared to
controls and other doses.
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Keywords
Bali cattle, cryopreservation, extract, moringa leaf, spermatozoa
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