FRAKSINASI EKSTRAK ETIL ASETAT DAN UJI KROMATOGRAFI LAPIS TIPIS DARI FERMENTASI KO-KULTUR Penicillium sp. LBKURCC34 DAN Staphylococcus aureus
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Date
2022-03
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Elfitra
Abstract
Penicillium sp. LBKURCC34 is a fungus that can produce bioactive secondary metabolites so
that it can be used as a candidate for antimicrobial compounds producer. This study aimed to
initiate separation of antimicrobial compounds from secondary metabolites of co-culture
fermentation Penicillium sp. LBKURCC34 and Staphylococcus aureus. The ethyl acetate extract
of the co-culture fermentation was obtained from liquid-liquid extraction of fermentation media
and ethyl acetate solvent. That crude extract was then fractionated using vacuum liquid
chromatography (VLC). Thin layer chromatography (TLC) test was performed on crude extract
and all fractions with ethyl acetate – n-hexane (7:3) and ethyl acetate – methanol (3:1) as eluents.
Fractionation of crude extract from vacuum liquid chromatography produced 15 fractions with
different spot patterns seen under 254 nm UV lamp.
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Keywords
antimicrobial, co-culture, secondary metabolites
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