ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli
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Date
2014-03-28
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Abstract
Garcinia mangostana Linn is one of the popular local plants that has been used as medicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactive
compounds from extract of mangosteen fruit husk were thought to produce by
endophytic fungi. The purpose of this research were to isolate and characterize
endophytic fungi from mangosteen fruit husk that potential to produce antimicrobial compound. Antimicrobial activity was determined by measuring the inhibition zone with Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candida albicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolates
endophytic fungi which were obtained from mangosteen fruit husk were known to have
antimicrobial activities. Endophytic fungi producing antimicrobial belongs to genus Aspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungi having the highest antimicrobial activities in agar disc assay were Penicillium
sp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR and Aspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities in Kirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicillium
sp.1KMA was significantly different with the other four endophytic fungi
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Keywords
Candida albicans, Escherichia coli, endophytic fungi, mangosteen, Staphylococcus aureus