AKTIVITAS ANTIBAKTERI ISOLAT AKTINOMISETES DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli
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Date
2022-04
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Elfitra
Abstract
The use of antibiotics for infections treatment in the long term may cause bacteria
become resistant. Exploration of microorganisms capable to produce antimicrobial
compounds have widely been reported. One of which is actinomycetes, a gram-positive
bacteria that produce various secondary metabolites. The purpose of this study was to
test and select the ability of 20 isolates of actinomycetes (14 isolates from the
rhizosphere of the Siak-siak plant (Dianella ensifolia) and six isolates from mangrove
soil in Kuala Enok, Tanah Merah district, Indragiri Hilir) using plug agar diffusion
method against E.coli under Mueller Hinton Agar (MHA) medium for 24 hours. Two
isolates (RK4 and RW5) formed inhibition zones against E.coli with diameters of 8 mm
and 10.5 mm, respectively. Based on the results obtained, the two isolates were
classified as bacteriostatic. In addition, inhibition capability of RK4 and RW5 isolates
were respectively classified into weak and moderate inhibitory criteria.
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Keywords
Actinomycetes, Antimicrobial, Bacteriostatic, Plug Agar diffusion method
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