Abstract:
Gliocladium sp. TNC73 and TNC59 are effective biocontrol fungi isolated
from Riau soil. These strains have been tested for their effectiveness in plant
protection through antagonistic studies and plant protection studies. The ability of
Gliocladium sp. TNC73 and TNC59 lies among others to their ability in
producing fungal cell wedl degrading enzymes. Apart from chitin, P-1,3 glucan
and P-1,6 glucan are among the major fimgal cell wall constituents in fungal
phatogens.
Analysis of laminarinase produced from Gliocladium sp. TNC73 and
TNC59 are very important to apply these biocontrol fungi effectively, to know the
kinds of specific fungal plant pathogens that they inhibit. Apart from p-1,3 glucan
and P-1,6 glucan linkage hydrolysis, laminarinase have tlie potential to be used in
developement of pharmaceuticals. Laminarinase is used to modify complex
polysaccharidee used as anticancer drugs.
Laminarinase from Gliocladium sp. TNC73 and TNC59 were produced in
production media containing 0,2 % laminarin from laminaria digitata, pH 5,5,
room temperature, and monitored over time. Activity of laminarinase was
determined by incubation of crude extracts with 0,2 % laminarin for 1 hour at
40"C, pH 5,5. One unit (U) of activity was defined as the quantity of enzyme that
released 1 ^mol reducing sugar per minute. The highest laminarinase activity was
produced by Gliocladium sp. TNC73 with an activity of 0,058 U/mL crude
enzyme after 7 days of production. This is significantiy higher (p<0,05) than that
produced by Gliocladium sp. TNC59 with an activity of 0,018 U/mL. The specific
activity of laminarinase is significantly higher (p<0,05) than Gliocladium sp.
TNC73 and Gliocladium sp. TNC59.
