Isolasi Dan Karakterisasi Bakteri Penghasil Poli Hidroksialkanoat (Pha) Sebagai Bahan Baku Plastik Biodegradabel Dari Kawasan Taman Hutan Raya Sultan Syarif Hasyim Provinsi Riau

Abstract

Plastic garbage has become a global scjile of environmental problem because plastic cannot be waved by environment. The development of biodegradable plastic stuff is one of alternative to solve this problem. Poli-l3-hidroksialkanoat (PHA) is one of element substance compound from biodegradable plastic stuff which is produce by granule in a cell. The purpose of this research is to isolation of amylolitic bacteria which can produce PHA as biodegracUible plastic from Great Forest Garden Area of Sultan Syarif Hasyim in Riau Province. There are 21 isolate bacteria have successfully isolated from land sampel that taken from Great Forest Garden Area of Suhan Syarif Hasyim in Riau Province. Bacteria Isolation of land sample was conducted by using starch hydrolysis medium. The characterization which are classiflcated based on the ability in degradation of amylum was indicated by observing of clear zone around the colony, qualitatively result 12 isolate bacteria and verified by iodium staining. Analysed qualitatively by staining with Sudan Black B, xylene and safranin, twelfth isolate able to accumulate or produce PHA with observed as a black granule in a bacteria cell were examined under phase contrast microscope at lOOOx magnification. These Isolates have prospects in industry of biodegradable plastic from starch substrat production