Abstract:
Pulai basung (Alstonia spatulata Bl) is a type of tree that is widely spreaded throughout
Indonesia with medicinal content in it. This plant is included in the genus Alstonia and
the Apocynaceae family. The purpose of this study was to analyze the toxicity test of
the dichloromethane fraction pH 9 of A. spatulata stem bark using the brine shrimp
lethality test (BSLT) method. This study used acid-base extraction method. Samples
were extracted by maceration method using methanol solvent and partitioned using
chloroform (pH 8) and dichloromethane (pH 9). The results of the KLT chromatograms
of the two fractions showed the same number of stains on both extracts. In this study,
the isolation process was continued using the dichloromethane fraction pH 9 while the
toxicity test was continued on the chloroform fraction pH 8. The results of the toxicity
test using the BSLT method showed that the chloroform fraction pH 8 was toxic with an
LC50 value of 292.32 ppm.
