Abstract:
Tetrazoloquinazoline or tetrazolopyrimidine is a nitrogen-containing six-ring heterocyclic
compound and a tetrazole ring which are pharmacologically important structural molecules with
broad bioactivity as antidiabetic, anticancer, and antimalarial. This research aims to synthesize
tetrazoloquinazoline derivatives and determine their bioactivity. Qz-4Cl synthesized by
multicomponent reaction (MCR) between 5-aminotetrazole, 4-chlorobenzaldehyde, and 1,3-
cyclohexadione used p-toluen sulphonic acid (p-TSOH) catalyst under solvent-free conditions.
ET-4Cl-Qz was synthesized by substitution reaction between Qz-4Cl with ethyl chloroacetate used
potassium carbonate (K2CO3) catalyst in dimethylformamide (DMF) solvent. The purity of the
compounds was determined by the TLC test, melting point measurement, and HPLC. The structure
of the synthesized compounds was confirmed by spectroscopic analysis of UV, FTIR, 1H-NMR,
13C-NMR, and HRMS. The toxicity of ET-4Cl-Qz was tested by the Brine Shrimp Lethality Test
(BSLT) method used by Artemia salina Leach. ET-4Cl-Qz was toxic with an LC50 value of 25.23
μg/mL.
