Abstract:
The use of cellulose from plants for paper production continuously requires energy
and can damage the environment, so an alternative source of renewable cellulose is needed
with more efficient results, namely the production of cellulose from bacteria. Therefore,
this study aimed to isolate and test cellulose-producing bacteria from mangosteen, durian,
kapulasan and industrial rubber waste for paper production applications. The method used
was the isolation of cellulose bacteria using the Hestrin Schramm (HS) medium, the
selection of selected isolates using the Glucose Extract Yeast (GEY) medium, then A
production test was carried out using a modified coconut water medium under static
conditions for 14 days and the determination of bacterial cellulose production. The results
showed that as many as 6 isolates (MSB-1b, MSB-2b, MSB-5b, PULR-1b, PULR-2b and
PULR-2c) that were tested were successful in forming cellulose mats and none were
successful in forming cellulose pellicles. The z / k ratio values of each isolate were MSB-1b
= 2.15, MSB-2b = 2.70, MSB-5b = 2.85, PULR-1b = 5.93, PULR-2b = 5.30 , and PULR-2c
= 5.10. Based on the research results, the z / k ratio cannot be used as a reference in
selecting the ability of cellulose bacteria to form pellicles, so it cannot be known about its
ability as a material for paper production.
