Abstract:
Efforts to obtain plant cellulose require energy and can damage the environment,
so an alternative source of cellulose that can be renewed with a more efficient result is
bacterial cellulose. For this reason, this study aims to obtain bacterial isolates that have the
potential to produce cellulose pellicles which can be applied in the textile fiber industry.
The sample was grown on hestrin-schramm medium and incubated. The cellulose mat
formed was then gradually diluted and inoculated into the glucose ethanol and yeast extract
medium. The isolates that produced the clear zone were then purified on glucose ethanol
and yeast extract medium to measure the Z/K ratio. Then the production is carried out using
a modified production medium with the addition of coconut water. The cellulose pellicle
formed was then purified and the dry weight and moisture content were calculated. At the
time of isolation, only grape and orange samples could produce cellulose mats, while peat
soil samples were unable to form cellulose mats and cellulose pellicles. At the time of
selection, 8 isolates were obtained from grapes and 5 isolates from citrus fruits that
produced clear zones. However, at the time of production, only A8 isolates from grapes
were able to produce cellulose pellicles. The resulting cellulose pellicle is thick, dense and
white in color.
