Browsing by Author "Wati, Laras"

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    PENGARUH PENAMBAHAN EDTA DAN SDS TERHADAP AKTIVITAS ENZIM LAKASE B Trichoderma asperellum LBKURCC1
    (Elfitra, 2023-12) Wati, Laras; Dahliaty, Andi; Nugroho, Titania Tj
    Laccase enzyme is an extracellular enzyme that is catalyzing lignin degradation process. This study aims to analyze the effect of the addition of EDTA and SDS compounds on laccase enzyme activity with concentration variations of 1 mM and 10 mM. Laccase enzyme from Trichoderma asperellum LBKURCC1 had been partially purified by 0-80% ammonium sulfate fractionation and gel filtration chromatography where the results of this partial purification produced two kinds of laccase, namely laccase A and laccase B. This study used laccase B enzyme. The activity of laccase B enzyme was determined by UV-Vis spectrophotomoter at 420 nm wavelength using ABTS (2,2'-Azinobis-3-ethylbenzothiazolin-6- sulfonate) as substrate. The activity of laccase B enzyme was 144.02±2.71 U/L. Addition by 1 mM EDTA was not significantly different (p>0.05) with relative activity of (100.47±6.47)% control. A significant decrease in laccase activity (p≤0.05) occurred in the addition by EDTA 10 mM, SDS 1 mM and SDS 10 mM with relative activities of (76.58±2.6)%, (38.81±3.59)%, (8.98±1.35)% of the control, respectively.