Browsing by Author "Pratiwi, Nova Wahyu"

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    ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI KULIT PISANG MANIS (Musa acuminata)
    (Elfitra, 2022-07) Amelia, Savini Rezki; Pratiwi, Nova Wahyu
    Lactic acid bacteria (LAB) are bacteria that produce lactic acid. LAB in general can live naturally in vegetables and fruits including banana peels. Banana peel is rich in carbohydrates so that it can be a substrate for LAB growth. This study aimed to isolate and characterize LAB from the peel of sweet banana (Musa acuminata). Isolation of LAB from banana peels was carried out using the spread plate method using Man Rogosa Sharpe Agar selective medium. All morphological characterization, physiological and biochemical tests were investigated. The results of the isolation obtained 5 LAB isolates. Based on the colony morphological test, all isolates were Gram-positive bacteria. They showed variation based on physiological and biochemical tests.
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    ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI PEPAYA (Carica papaya L.)
    (Elfitra, 2022-07) Nurhakiki, Nurhakiki; Pratiwi, Nova Wahyu
    Papaya fruit (Carica papaya L.) is a potential source of lactic acid bacteria (LAB). LAB produces various metabolites that have the potential to be used as probiotic agents. This study aimed to isolate LAB from papaya fruit in order to determine the phenotypic characters. LAB suspension isolated from california papaya was inoculated into de Man Rogosa and Sharpe Agar (MRSA) medium. Subsequently, the LAB suspected isolates were phenotypically characterized based on colony morphology and biochemical properties. A total of 5 LAB isolates obtained were creamy and milky white, Gram positive, coccobacillus shaped with catalase negative, and homofermentative fermentation to glucose.
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    ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI AKTINOMISETES DARI ARBORETUM UNIVERSITAS RIAU
    (2017-01-10) Rahayu, Fatimah; Roza, Rodesia Mustika; Pratiwi, Nova Wahyu
    Demand of antibiotics for phatogenic bacteria is getting increased, therefore, the discovery of novel antibiotics is needed to address antibiotics resistancy problem. Actinomycetes are well known to produce secondary metabolites such as antibiotics. Isolation of actinomycetes was conducted by making initial treatment of 4 soil samples with dry heat and wet heat to inoculate on Starch Casein Agar (SCA). From 4 sampled location at Univesitas Riau Arboretum, Location 3 had the most various of actinomycetes discovered. Wet heat treatment gave higher number of isolated actinomycetes with a number of 23 isolates. In this study, total actinomycetes isolated from samples were 30 isolates and tested their antibacterial activity. Actinomycetes isolate code C2.20 had the widest clear zone of 21.29 mm against pathogenic bacteria Staphylococcus aureus with agar disc method.