Browsing by Author "Muria Sri Rezeki"

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    PENGARUH KONSENTRASI SUBSTRAT DAN WAKTU FERMENTASI PADA PEMANFAATAN LIMBAH KULIT NENAS (ANANAS COMOSUS L.MERR) UNTUK PRODUKSI ENZIM SELULASE
    (2013-01-09) Pasaribu, Fitri Lasmini; Yenie Elvi; Muria Sri Rezeki
    Pineapple’s skin is a fairly large agricultural wastes in Indonesia. Potential cellulose in a large pineapple’s skin can be used as a substrate in the production of cellulases that can add economic value to the pineapple’s skin. The purpose of this research was to produced cellulase enzyme and determine the effect of fermentation time and substrate concentration on enzyme production. The method in this research used solid fermentation system. The research did by fixed variables such as nutrient in the media (urea 30 mg, MgSO4.7H2O 5 mg, 2.3 mg KH2PO4), the beginning pH fermentation used pH 5, fermentation temperature used room temperature. The change variables used substrate concentration 0.5 g / ml, 1 g / ml, 1.5 g / ml, 2 g / ml and a variable fermentation time 2 days, 3 days, 4 days, 5 days, 6 days. Fermentation’s result was extracted and centrifuged to obtain a crude extract of cellulose enzyme. Then the crude extract of cellulase enzyme activity was tested by adding cotton and glucose hydrolysis yield was determined using DNS (3,5-dinitrosalicylic acid). The experimental results showed that the substrate pineapple’s skin can be used to produce the enzyme cellulase by Aspergillus niger with the highest enzyme activity were seen from the highest glucose 3.57 gr glucose/liter. Optimum enzyme activity obtained at substrate concentration 1 gr/ml and 4 days of fermentation time.
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    PENGARUH TEMPERATUR LIKUIFIKASI KONVERSI PATI SORGUM MENJADI GULA dan WAKTU SIMULTANEOUS SACCHARIFICATION and FERMENTATION (SSF) PRODUKSI BIOETANOL
    (2013-01-14) Masyithah, Mahfirani; Chairul; Muria Sri Rezeki
    Sorghum is one of the potential materials in the manufacture of bioethanol. Sorghum has advantages over sugarcane and maize, that is a shorter harvest time, needs little water and a source of ethanol can be obtained from the sap, starch and pulp. The purpose of this study to determine the effect of temperature liquefications of sugar produced and determine the best fermentation time. Part of sorghum is used as raw material for bio-ethanol is grain sorghum containing 73% carbohydrate by immersion NaOH to remove proteins in grain sorghum that can inhibit the action of enzymes. The process used is liquefications and simultaneous saccharification and fermentation by varying the temperature of liquefications, that is 75 °C, 85 °C and 95 °C and Simultaneous Saccharification and Fermentation time is 12, 24, 48 and 72 hours. Fermentation was carried out with the help of yeast Saccharomyces cerevisiae. Tests were conducted with alkoholmeter and sugar analysis using visible spectrophotometer results showed that the highest sugar yield is 4.685 g/l at a temperature of liquefications 95 °C and the highest ethanol concentration of 70 g/l at 72 hours with the temperature fermentation liquifikasi 95 °C