Browsing by Author "Jose, Christine"

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    CHANGES IN PHENOLIC AND FLAVONOID CONTENT OF Coleus amboinicus TEA DURING STORAGE
    (2014-05-21) Jose, Christine; Nurhayati; Chainulfiffah
    Coleus amboinicus plants were grown organically treated with three different foliar treatments (control, herb fermented plant extract and EM5). Green tea and black tea were produced from each treatment and stored for up to three months. All green teas have significant higher (P<0.05) phenolic and flavonoid contents compared to all black teas. Green tea produced from EM5 foliar treatment showed the highest phenolic and flavonoid content. Some changes of phenolic and flavonoid contents were observed in green teas and black teas during 3 months storage.
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    Optimalisasi produksi enzim selulase isolat selulolitik yang diisolasi dari Sungai Siak
    (2013-04-16) Haryani, Yuli; Jose, Christine; dahliaty, andi; devi, silvera
    Isolates of S-16 and S-22 are some of the local strains producing cellulases that have been isolated from the Siak River water. Cellulase enzyme can be degrades of cellulosewaste containing that enable for the processing of waste to economically priced products such as glucose. In this research, optimization of the production of cellulase enzymes from isolates of S-16 and S-22 with a variety of media composition, temperature and concentration of carbon source production. The quantitative assay for enzyme activity was determined by Nelson Somogyi method with carboxymethyl of cellulose (CMC) as a substrate. The highest cellulase enzyme activity of S-16 obtained in a media production (KH2P04; MgS04.7H20; NaN03; CaC12; NaCl; CMC) of 88x10-3 U / mL, and the optimum concentration of source of carbon at 1% obtained for 50.36 xlO-3 U / mL, whereas of S-22 obtained in a media production (KH2P04; MgS04.7H20; FeS04.7H20; NaN03; KCI; CMC) of 14x10-3 U / mL, and the optimum concentration of sources of carbon at 1% obtained for 14 , 7x10-3 U / mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test multiple range at level 5%.
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    PENENTUAN FENOLIK, VITAMIN C DAN AKTIVITAS ANTIOKSIDAN TANAMAN SELADA (Lactuca sativa L.) YANG DIRAWAT DENGAN EKSTRAK TANAMAN TERFERMENTASI
    (2012-11-20) Reno Raisandi, Mohd; Jose, Christine; Hendra, Rudi
    Lettuce (Lactuca sativa L) can serve as a source of vitamins, minerals and natural antioxidants. The cultivation system organically and conventionally can influence the nutrients and antioxidant contents. In this study, the total phenolic, flavonoid, vitamin C content, antioxidant activity and flavonoid composition of lettuce extract were carried out. Fresh weight and percentage of damaged leaves of lettuce were determine too as physical analysis. Two different treatment (FPE and control) were used for organic lettuce. The results showed that the fresh weight of plants were not significantly different. Organic lettuce with FPE treatment (5,10%) had lower (P0,05) percentage of leaves damaged than control treatment (18,23%). Compared to conventional lettuce, organic lettuce with FPE (9,873 GAE mg/g) and control (9,000 GAE mg/g) treatment had a significantly (P0,05) higher content of phenolic. Organic lettuce with FPE treatment showed higher flavonoid (10,681 QE mg/g) and vitamin C (0,460 AAE mg/g) content. For antioxidant activities, organic lettuce with FPE treatment gave the best FRAP (0,234 mmol Fe2+/g), FTC (55,05%), DPPH (482,33 g/mL) and NO radical scavenging (4,619 mg/mL) values. Determination of flavonoid composition showed that lettuce extracts didn’t contain standards of flavonoid compound (quercetin, catechin, naringin, and rutin). Lettuce extracts could be contained other flavonoid compounds. From this study, it can be concluded that the percentage of phenolic and vitamin C might be contributed to the antioxidant activity and the amount of total phenolic and vitamin C content which might be influenced by the cultivation system.