Browsing by Author "Fitriyanti"
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Item Degradasi Pewarna Azo Mordant Black 17 oleh Ganoderma sp. BTA1 Strain Lokal(wahyu sari yeni, 2017-09-07) Martina, Atria; Rahayu, Lila Kusuma; FitriyantiGanoderma sp. BTA1 local strain is a white rot basidiomycetes that have ligninolytic activities. This fungi was used for azo Mordant Black 17 (MB17) degradation. Their performance were evaluated at different Mordant Black 17 concentration and incubation time. To approve the ability of those isolates using N-limited medium and monitoring with specrofotometer. Idiophase of this isolate was 11th day. The experiment on MB 17, indicated that the Ganoderma sp BTA1 were resistent at 200 mg/l concentration of MB 17 with optimal result 150 ppm. The optimal concentration was used for further experiment. The result of this study indicated that the capacity of MB17 degradation was affected by incubation times. The degradation rate was greater at six days after idiophase with 95%.Item OPTIMALISASI pH PRODUKSI ENZIM AMILASE BAKTERI ENDOFITIK Pseudomonas stutzery LBKURCC53(2016-10-19) Fitriyanti; Devi, Silvera; SaryonoPseudomonas stutzery LBKURCC49, LBKURCC51 and LBKURCC53 are local endofitic bacteria that can produce amylase. Amylase is an enzyme which hydrolyze amilum into maltose and glucose by breaking down the α-1,4 glycosidic linkages. The production of amylase enzyme from microorganisms can be optimized by adjusting the fermentation conditions. The activity of amylase enzyme was determined with the DNS (dinitrosalicylic) methods. The highest amylase enzyme activity at pH 6.0, 40°C and 120 rpm was shown by Pseudomonas stutzery LBKURCC53. Furthermore, optimalization of amylase production by LBKURCC53 was conducted performed at various pHs (6.5; 7.0; 6.0; 7.5). The results showed that the optimum amylase enzyme activity was 66.173 x 10-3 U/mL at pH 6.0