6.International Seminar on Fisheries and Marine Science 2015

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Browsing 6.International Seminar on Fisheries and Marine Science 2015 by Author "Nur, Indriyani"

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    The Effect of Antibiotics in the Hatching of Eggs of Ornated Lobster (Panulirus ornatus)
    (2016-08-28) Yusnaini; Nur, Indriyani
    The outbreak of micro organism infection is used to controlled using antibiotics. As lobsters spawn externally, their eggs were easily infected by microorganisms. To understand the effects of antibiotics in the hatching of P.ornatus eggs, a study has been conducted by removing the eggs from from berried spiny lobsters. The eggs were then treated using two types of antibiotics; Oxytetracyclin (2, 4, 6, and 8 ppm ) and Erythromycin (10, 20, 30, and 40 ppm). The control was not treated with antibiotic. Eggs were then incubated artificially in a recirculation system and monitored at 12, 24, 30, 36, 42, 64, and 88 hours for hatching rate (HR). Results shown that the Oxytetracyclin (6 ppm) and Erythromycin (40 ppm) result significant increase in HR, they were 20% and 18.67%, respectively. Data obtained indicate that antibiotics treatment could be a strategy for increasing the HR of lobster eggs.
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    Phylogenetic and Structure Comparative Analysis of Complement Regulatory Membrane Proteins of Ginbuna Crucian Carp Carassius auratus langsdorfii
    (2016-08-28) Nur, Indriyani; Harada, Hikari; Nakamura, Ryota; Tsujikura, Masakazu; Somamoto, Tomonori
    Regulation of the complement activation (RCA) has been shown to play a key role in controlling activation of the complement cascades as the main part of innate immune system. Humans RCAs have been studied most extensively and classified into soluble and membrane type. RCAs have also been partly characterized in a limited variety of non-mammalians species, including in bony fish, such as carp and zebrafish. In recent year, we have found three isoforms of complement regulatory membrane proteins (Tecrem) in ginbuna crucian carp at mRNA level, namely gTecrem-1, gTecrem-2, and gTecrem-3 in which structurally homologous to mammalian RCA-membrane type (CD46). In this study, phylogenetic analyses and comparative study on gTecrem structure were performed to clarify fundamental molecular information. Data based on mRNA level indicated that gTecrem structure principally conserved all the features of non-mammalians and human membrane-bound RCA. In addition, the findings showed that the number of Short Concensus Repeat (SCRs) in regulatory membrane-bound protein is different across isoforms and animal spesies.