OPTIMASI WAKTU PRODUKSI METABOLIT SEKUNDER AKTINOMISETES ASAL TANAH KEBUN SAYUR DI KOTA PEKANBARU: UJI DAYA HAMBAT PERTUMBUHAN Staphylococcus aureus

Abstract

Actinomycetes is a gram-positive bacteria that produce various secondary metabolites such as antibiotics. Secondary metabolites can use to control microbial pathogen such as Staphylococcus aureus. Actinomycetes isolates had been isolated from Vegetable Garden Soil of Pekanbaru city namely isolates M1.T1, M1.T3, M1.T4 and M1.T5. The purpose of this research is to determine the optimal secondary metabolite time used by actinomycetes isolate from Vegetable Garden Soil to inhibit the grows of Staphylococcus aureus. Optimization of secondary metabolites production time was done by incubation until 14 days. The test method used to examine antibacterial activity was disc diffusion method. The optimum incubation period in producing secondary metabolite in isolate actinomycetes against Staphylococcus aureus is on the M1.T1 isolated the 3rd day incubation with inhibiton of 18,8±0,1 mm, on the M1.T3 isolated the 8th day incubation with inhibition of 12,3±0,2 mm, on the M1.T4 and M1.T5 isolated the 9th day incubation with each inhibition of 10,6±0,2 and 18,8±0,1 mm. The diameter of the inhibition zone formed was compared with the tetracylcline and produced antibacterial activity criteria of actinomycetes isolates that were resistant, intermediate and sensitive.