Abstract:
Actinomycetes is a gram-positive bacteria that produce various secondary
metabolites such as antibiotics. Secondary metabolites can use to control microbial
pathogen such as Staphylococcus aureus. Actinomycetes isolates had been isolated from
Vegetable Garden Soil of Pekanbaru city namely isolates M1.T1, M1.T3, M1.T4 and
M1.T5. The purpose of this research is to determine the optimal secondary metabolite
time used by actinomycetes isolate from Vegetable Garden Soil to inhibit the grows of
Staphylococcus aureus. Optimization of secondary metabolites production time was
done by incubation until 14 days. The test method used to examine antibacterial activity
was disc diffusion method. The optimum incubation period in producing secondary
metabolite in isolate actinomycetes against Staphylococcus aureus is on the M1.T1
isolated the 3rd day incubation with inhibiton of 18,8±0,1 mm, on the M1.T3 isolated the
8th day incubation with inhibition of 12,3±0,2 mm, on the M1.T4 and M1.T5 isolated the
9th day incubation with each inhibition of 10,6±0,2 and 18,8±0,1 mm. The diameter of
the inhibition zone formed was compared with the tetracylcline and produced
antibacterial activity criteria of actinomycetes isolates that were resistant, intermediate
and sensitive.
