ISOLASI DAN UJI TOKSISITAS SENYAWA METABOLIT SEKUNDER EKSTRAK n-HEKSANA DAUN NONA MAKAN SIRIH (Clerodendrum thomsoniae Balf.f)

Abstract

Nona makan sirih (Clerodenrum thomsoniae) is a medicinal plant from the family Lamiaceae, and the genus Clerodendrum. It is usually grows as an ornamental plant. This study aimed to isolate and characterize the pure compound from the n-hexane extract of the nona makan sirih leaf plant (Clerodendrum thomsoniae) and to analyze a its toxicity of the isolated compound and n-hexane extract of the nona makan sirih leaf (Clerodendrum thomsoniae) using the brine shrimp lethality test (BSLT) method. Nona makan sirih was macerated using methanol as a solvent and then fractionation using n-hexane. The separation of compounds was carried out by vacuum liquid chromatography (VLC) and obtained 12 fractions. In fraction 2, there was one spot on the TLC plate which indicated this fraction was pure and coded as CT-H2. The purity test of CT-H2 was analyzed by thin layer chromatography test (TLC) with several eluents mixtures. CT-H2 were characterized using a FT-IR spectrophotometer. The results of the phytochemical characterization and analysis of the CT-H2 compound showed some characters of terpenoid. Toxicity test showed the LC50 values of CT-H2 and n-hexane fraction were 254.22 μg/mL and 209.79 μg/mL, respectively. The LC50 value <1000 μg/mL indicates that the n-hexane fraction and CT-H2 have potential activity be developed as a cytotoxic agent.