ISOLASI SENYAWA METABOLIT SEKUNDER DAN PENGHAMBATAN α-GLUKOSIDASE DARI EKSTRAK n-HEKSANA TANAMAN MIANA HIJAU (Coleus hybridus)

Abstract

Miana (C. hybridus) is an ornamental plant that is widely used as a medicinal plant. This study aimed to isolate secondary metabolites and test α-glucosidase inhibition from these plants. Isolation was carried out by maceration method with methanol and then partitioned with n-hexane solvent to obtain a thick n-hexane extract. Extracts were separated by vacuum liquid chromatography (VLC) and purified by flash chromatography. Two isolated pure compounds were obtained codenamed CHG-H7 and CHG-H8 with melting points of 134º-136ºC and 136º-138ºC, characterized using FTIR spectroscopy. α-Glucosidase inhibitory test was carried out using the α-glucosidase enzyme and acarbose as positive controls. The results, it is known that the n-hexane extract of C. hybridus has an IC50 value of 452.62 μg/mL with acarbose having an IC50 value of 68.410 μg/mL. The comparison of the IC50 value which is quite high between the extract and acarbose can occur because in the tests carried out, the acarbose used is a pure compound. Based on the results of the research that has been done, it can be concluded that the n-hexane extract of C. hybridus has the potential as an enzyme inhibitor α-glucosidase