Abstract:
Miana (C. hybridus) is an ornamental plant that is widely used as a medicinal plant.
This study aimed to isolate secondary metabolites and test α-glucosidase inhibition from
these plants. Isolation was carried out by maceration method with methanol and then
partitioned with n-hexane solvent to obtain a thick n-hexane extract. Extracts were
separated by vacuum liquid chromatography (VLC) and purified by flash
chromatography. Two isolated pure compounds were obtained codenamed CHG-H7 and
CHG-H8 with melting points of 134º-136ºC and 136º-138ºC, characterized using FTIR
spectroscopy. α-Glucosidase inhibitory test was carried out using the α-glucosidase
enzyme and acarbose as positive controls. The results, it is known that the n-hexane
extract of C. hybridus has an IC50 value of 452.62 μg/mL with acarbose having an IC50
value of 68.410 μg/mL. The comparison of the IC50 value which is quite high between
the extract and acarbose can occur because in the tests carried out, the acarbose used is a
pure compound. Based on the results of the research that has been done, it can be
concluded that the n-hexane extract of C. hybridus has the potential as an enzyme
inhibitor α-glucosidase
