BAKTERI PENGHASIL BIOPLASTIK DARI LIMBAH INDUSTRI SAGU DAN TAHU DI RIAU: ISOLASI, SELEKSI, DAN OPTIMASI PRODUKSI

Abstract

Conventional plastic is difficult to degrade and can pollute the environment. Therefore, an alternative to conventional plastic is needed by using bioplastics that are more easily biodegradable. The purpose of this study was to obtain potential bacterial isolates to produce PHA as raw material for bioplastics from industrial waste of sago and tofu in Riau. PHA-producing bacteria were isolated using Nutrient Agar medium using the spread plate method. Qualitatively, PHAproducing bacteria were selected based on their ability to absorb the Sudan black solution. The selected bacterial isolates were cultured in mineral salt medium (MSM) for the production of PHA and the resulting PHA was extracted using chloroform. A total of 5 isolates that showed high color absorption intensity were isolated from sago factory waste located in Goguk Village, Meranti Islands. The highest PHA accumulation was produced in 50% palm oil mill liquid waste (LCPKS) medium by LPS1_9 isolate, while the lowest PHA accumulation was produced in MSM medium by LPS1_12 isolate. The use of 50% LCPKS as a carbon source tends to support an increase in the accumulation of PHA, compared to the use of a carbon source from a minimal nutrient medium MSM.