Abstract:
Conventional plastic is difficult to degrade and can pollute the environment.
Therefore, an alternative to conventional plastic is needed by using bioplastics
that are more easily biodegradable. The purpose of this study was to obtain
potential bacterial isolates to produce PHA as raw material for bioplastics from
industrial waste of sago and tofu in Riau. PHA-producing bacteria were isolated
using Nutrient Agar medium using the spread plate method. Qualitatively, PHAproducing
bacteria were selected based on their ability to absorb the Sudan black
solution. The selected bacterial isolates were cultured in mineral salt medium
(MSM) for the production of PHA and the resulting PHA was extracted using
chloroform. A total of 5 isolates that showed high color absorption intensity were
isolated from sago factory waste located in Goguk Village, Meranti Islands. The
highest PHA accumulation was produced in 50% palm oil mill liquid waste
(LCPKS) medium by LPS1_9 isolate, while the lowest PHA accumulation was
produced in MSM medium by LPS1_12 isolate. The use of 50% LCPKS as a
carbon source tends to support an increase in the accumulation of PHA,
compared to the use of a carbon source from a minimal nutrient medium MSM.
