Abstract:
Petroleum production activities can cause environmental pollution which
has a major impact on humans and the ecosystem. One of the efforts to polluted
environmental conditions is by biodegradation. The research aimed to determine
the growth ability of Bacillus sp. with immobilization techniques using oil palm
bunches (TKKS), sawdust (SG) and rice husks (SP) carriers to degrade petroleum.
The growth ability of bacterial isolates was tested on Minimal Salt Medium
(MSM) + 1% crude oil and pH was measured for a period of 0, 5, 10 and 15 days.
The results showed that the highest total population of Bacillus Sp 48 bacteria on
the 10th day of TKKS treatment was 2,67x109 cfu/ml and SG 2,58x109 cfu/ml and
growth test of Bacillus Sp 48 gave the highest population by giving TKKS carrier
on the 10th day. Bacillus Sp 48 had the potential to be developed as a
bioremediation agent.
