ISOLASI BAKTERI PENGHASIL POLIHIDROKSI ALKANOAT (PHA) DARI LIMBAH CAIR PABRIK KELAPA SAWIT DAN TANAH MANGROVE RIAU

Abstract

Plastic waste that is difficult to degrade becomes a serious problem because it can pollute the environment. Therefore, it is necessary to be handled with the use of bioplastics or environmentally friendly plastics that will be decomposed by microorganisms. The purpose of this research was to obtain potential isolates in producing Polyhydroxy Alkanoate (PHA) for bioplastics manufacture from the liquid waste of palm oil mills and Riau mangrove soils. PHAproducing bacteria were isolated using nutrient agar medium with spread method. Insulated bacteria were selected using Sudan Black B dye and bacteria that had the ability to produce PHA became dark blue or blackish blue. Selected bacterial isolates were cultured in mineral salt medium for PHA production. PHA produced was extracted using hot chloroform solvents. PHAproducing bacteria isolated from 3 sampling locations amounted to 65 bacterial isolates with 17 reselected bacterial isolates and 8 selected bacterial isolates conducted production tests. A total of 8 PHA-producing selected bacterial isolates had dry cell weight between 0.23-0.77 g/L, PHA levels of 0.004-0.12 g/L, biomass residues of 0.22-0.66 g/L and PHA accumulation percentages of 0.88-25.22%. The highest percentage of PHA accumulation was obtained in MTL1.1 isolates (25.22%) and the lowest was MTL3.2 isolate (0.88%), which came from mangrove soil samples of Tanjung Leban Village