Abstract:
Plastic waste that is difficult to degrade becomes a serious problem because it can pollute the
environment. Therefore, it is necessary to be handled with the use of bioplastics or
environmentally friendly plastics that will be decomposed by microorganisms. The purpose of
this research was to obtain potential isolates in producing Polyhydroxy Alkanoate (PHA) for
bioplastics manufacture from the liquid waste of palm oil mills and Riau mangrove soils. PHAproducing
bacteria were isolated using nutrient agar medium with spread method. Insulated
bacteria were selected using Sudan Black B dye and bacteria that had the ability to produce PHA
became dark blue or blackish blue. Selected bacterial isolates were cultured in mineral salt
medium for PHA production. PHA produced was extracted using hot chloroform solvents. PHAproducing
bacteria isolated from 3 sampling locations amounted to 65 bacterial isolates with 17
reselected bacterial isolates and 8 selected bacterial isolates conducted production tests. A total
of 8 PHA-producing selected bacterial isolates had dry cell weight between 0.23-0.77 g/L, PHA
levels of 0.004-0.12 g/L, biomass residues of 0.22-0.66 g/L and PHA accumulation percentages
of 0.88-25.22%. The highest percentage of PHA accumulation was obtained in MTL1.1 isolates
(25.22%) and the lowest was MTL3.2 isolate (0.88%), which came from mangrove soil samples
of Tanjung Leban Village
