Abstract:
Durik-durik plant is categorized as a member of Syzygium genus based on DNA
barcoding analysis using intergenic spacer psbA-trnH, matK, rbcl, ITS and trnL-F
intergenic spacer. Polymerase Chain Reaction (PCR) is an in vitro DNA amplification
technique using a thermal cycler machine to amplify DNA fragments in a relatively short
time. PCR process is influenced by the PCR components and the annealing temperature.
This research was conducted to optimize annealing temperature of 748F/1318R primer pair.
Temperature optimization is analyzed in various annealing temperature. Result showed that
the optimum temperature for amplification of ndhF gene is 46.6°C and the amplificon
obtained was 600 bp.
