dc.contributor.author |
Fitriani, Ana |
|
dc.date.accessioned |
2021-07-15T03:19:03Z |
|
dc.date.available |
2021-07-15T03:19:03Z |
|
dc.date.issued |
2020-07 |
|
dc.identifier.other |
wahyu sari yeni |
|
dc.identifier.uri |
https://repository.unri.ac.id/handle/123456789/10028 |
|
dc.description.abstract |
Durik-durik plant is categorized as a member of Syzygium genus based on DNA
barcoding analysis using intergenic spacer psbA-trnH, matK, rbcl, ITS and trnL-F
intergenic spacer. Polymerase Chain Reaction (PCR) is an in vitro DNA amplification
technique using a thermal cycler machine to amplify DNA fragments in a relatively short
time. PCR process is influenced by the PCR components and the annealing temperature.
This research was conducted to optimize annealing temperature of 748F/1318R primer pair.
Temperature optimization is analyzed in various annealing temperature. Result showed that
the optimum temperature for amplification of ndhF gene is 46.6°C and the amplificon
obtained was 600 bp. |
en_US |
dc.description.provenance |
Submitted by wahyu sari yeni (ayoe32@ymail.com) on 2021-07-15T03:19:03Z
No. of bitstreams: 1
REPOSITORY ANA FITRIANI_compressed.pdf: 158949 bytes, checksum: 31caa4fd2b2ed045e5b053c091e01776 (MD5) |
en |
dc.description.provenance |
Made available in DSpace on 2021-07-15T03:19:03Z (GMT). No. of bitstreams: 1
REPOSITORY ANA FITRIANI_compressed.pdf: 158949 bytes, checksum: 31caa4fd2b2ed045e5b053c091e01776 (MD5)
Previous issue date: 2020-07 |
en |
dc.description.sponsorship |
Jurusan Biologi
Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau |
en_US |
dc.language.iso |
en |
en_US |
dc.subject |
Amplification |
en_US |
dc.subject |
durik-durik |
en_US |
dc.subject |
Syzygium sp |
en_US |
dc.subject |
Polymerase Chain Reaction |
en_US |
dc.title |
OPTIMASI SUHU ANNEALING UNTUK AMPLIFIKASI GEN ndhF PADA TUMBUHAN DURIK-DURIK (Syzygium sp.) |
en_US |
dc.type |
Article |
en_US |
dc.contributor.supervisor |
Roslim, Dewi Indriyani |
|