Skrining Bakteri Vibrio sp Penyebab Penyakit Udang Berbasis Teknik Sekuens 16S rDNA
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Date
2015-03-07
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Abstract
This research was conducted from May to July 2008 and aim to know the species of
Vibrio sp that infected shrimp (Penaeus monodori) based on sequence 16S.rDNA.
The Vibrio were isolated from shrimp, culture farm water, and sea water in Bantan
Air Village, Bengkalis Island. Isolation, morphological observation and biochemical
tests were analized in the Integrated Laboratory in Marine Science Program of
Fisheries and Marine Science Faculty, University of Riau. PGR {Polymerase Chain
Reaction) and sequencing DNA process were conducted in Gen Technology
Laboratory, Biotech Center, BPPT Serpong. Based on BLAST system treasured
through Gen Bank, the result shown that five species were found, namely Vibrio
alginolyticus starin FNS 08, Vibrio parahaemolyticus strain FNS C08, Vibrio harveyi
strain FNS BOS, Vibrio shiloni strain FNS DOS and Vibrio vulnificus strain FNS EOS.
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Skrining Bakteri, Teknik Sekuens 16S rDNA