Uji Aktivitas Anti jamur Dan Angka Cemaran Mikroba Ekstrak Dan Fraksinat Metanol Dari Bunga Kuning Tanaman Dahlia (Dahlia Variabilis)
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Date
2015-07-07
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Abstract
producers of antimicrobial compounds is of the Family Compositae. Plant dahlia
{Dahlia variabilis) is found in Compositae plants in upland areas. Dahlia plants
rich in carbohydrates such as inulin, fat and protein that is a primary
metabolite.
Extraction flower of dahlia (Dahlia variabilis) that yellow made with
methanol in order to obtain the total methanol extract as much as much as 52.341
g. Extract was separated by vacuum liquid chromatography (VLC) in order to
obtain eight fractions which were combined under the same Rf value on TLC
test.
Antifungal activity carried out using disc diffusion method. In this study
the results obtained for the antifungal activity of the fungus Candida albicans with
inhibition zone diameter to the concentration of 1% and 2% respectively obtained
of 0.93 mm and 0.86 mm.Tests conducted with microbial contamination rate
determination of total plate coxmt of fungi and bacteria, and determination of total
coliform. On The methanol extract obtained negative results. Dahlia flower
methanol extract did not contain pathogenic microorganisms and non-pathogenic
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Keywords
Dahlia variabilis, Compositae, Extraction, Candida albicans