Molecular Characteristics Of Vibrio Sp Causing Black Tiger Prawn (Penaeus Monodon) Disease In Sumatra And Java Shrimp Ponds By 16s Rdna Sequencing


Shrimp disease caused by Vibrio sp is one of the main limiting factors in the increasing production in shrimp farming. This disease may kill the shrimp and cause high loss in shrimp culture in South East and East Asia. Samples of ten (10) individual of a ten months cultured giant tiger prawn as well as liter pond water and sea waters were collected from shrimp ponds in Bengkalis Island, Sumatra. Samples of shrimps were also collected from Jepara shrimp ponds in Central Java and they were selected by looking at their behaviour and unhealthy physical characteristics. Amplification, sequencing and bioinformatics analysis of 16S rDNA to identify Vibrio species were conducted in Biotech Center, BPPT Serpong Banten. The results of DNA sequencing of each bacteria isolate were compared to DNA sequences from GenBank, the international DNA bank database. Tracing were made by BLAST (Basic Local Alignment Search Tool) system accessed through the internet at http://www./ The result of this study found that of seven strains of Vibrio sp. Bacteria analyzed, five were 97% homologues to V. alginolyticus, V. parahaemolyticus, V. harveyi, V. shilonii and V. vulnificus. Meanwhile, another two strains isolated in this study were not found to be homologues to any of the Gen-Bank listed strain and therefore are considered as indigenous Vibrio sp bacteria from Indonesia.



Vibrio sp, bacteria, shrimp, aquaculture, fish disease