Produksi Enzim Laminarinase Trichoderma Sp. Lokal Riau
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Date
2015-02-28
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Abstract
Trichoderma asperellum TNC52 and TNJ63 are two Riau Trichoderma sp.
biocontrol strain which can protect plants from several pathogen fungi. The
analysis of T. asperellum TNC52 and TNJ63 protection ability is very important
therefore the use of biocontrol more effective, to make sure the fungi activity was
inhibit. Beside degradate the y9-l,3 and y9-l,6 glucan bond, laminarinase also has
potential to develop in farmation industry, which was use to modificate the
protein-polysaccharide kompleks that nowdays use in cancer therapy.
Laminarinase T. asperellum TNC52 and TNJ63 were produce in media
contain laminarin 0,2%, pH 5,5, room temperature, the activity of laminarinase
was determinate by crude enzim extract incubation on with laminarin 0,02% for
hour, in 40"C temperature, pH 5,5. The research result of T. asperellum TNC52
show the activity of laminarinase crude extract was highest on 3-5 days
production with mean value (0,0102 ± 0,0005) unit/mL. The highest activity of
laminarinase TNJ63 crude extract was in 5 days production
(0,0090 ± 0,0064) unit/mL. Spesific activity of commersial Trichoderma sp.
enzyme was (3,6302 ± 0,8867) unit/mg protein which was significantly higher
(/7<0,05) than specific activity of crude enzyme laminarinase T. asperellum
TNC52 and TNJ63 extract, (0,9046 ± 0,1257) unit/mg protein and
(0,7480 ± 0,0616) unit/mg protein. While the activity of T. asperellum
laminarinase TNC52 and TNJ63 not different significantly (p>0,05) one another.
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Keywords
Trichoderma asperellum TNJ63, TNJ63, Trichoderma asperellum TNC52