SINTESIS DAN UJI TOKSISITAS ANALOG PIRAZOL 5-(2-FLUOROFENIL)-3-(NAFTALEN-1-IL)-1-FENIL-1H-PIRAZOL

Abstract

Pyrazole is five ring heterocyclic compounds with two adjacent nitrogen atoms and it have two endocyclic double bonds which is known to have diverse biological activities such as anti-inflammatory, analgesic, antibacterial, antimalarial, antioxidant, and anticancer. Pirazol analogue 5-(2-fluorofenil)-3-(naftalen-1-il)-1-fenil-1H-pirazol (PZL-2F) was synthesized via oxidative aromatization reaction of pyrazoline 5-(2-fluorofenil)-3-(naftalen-1-il)-1-fenil-4,5-dihidro-1H-pirazolin (PF CN1-2F) with glacial acetic acid. Synthesis of the pyrazole compound used the reflux at 85⁰C. The purity of the compound is determined by using TLC, melting point and HPLC analysis. The structure of the synthesized compound was confirmed by UV, FTIR, and GC-MS spectroscopic analysis. The yield of the synthesis PZL-2F compound was 57.7%. The toxicity of pyrazol was evaluated by Brine Shrimp Lethality Test (BSLT) method with shrimp larvae (Artemia salina Leach). Pyrazole PZL-2F had high toxicity with LC50 = 49,07 μg/mL.