PERBANYAKAN ANGGREK MACAN(Grammatophyllum scriptum (L.) Blume) SECARA IN VITRO DARI EKSPLAN TUNAS PADA MEDIA VACIN AND WENT

Abstract

Grammatophyllum scriptum (L.) Blume is an orchid species with threatened occurance in its natural habitat. In order to maintain the occurance of this species, it is necessary to conduct conservation efforts such as using in vitro culture technique. This study aimed to determine the best concentration of coconut water as well as its combination with BAP in the bud propagation of G. scriptum, using bud explants produced from in vitro culture. This study used a completely randomized design (CRD) with giving of various concentrations of coconut water treatment or in combination with BAP. The results showed that coconut water treatment or in combination with BAP could form buds and roots with a percentage of 100%. The coconut water treatment or in combination with BAP significantly affected the number of buds. The treatment of 1 mg/l BAP combined with 60% coconut water produced the highest number of shoots is (2.20).