INDUKSI TUNAS IN VITRO DARI TUNAS BATANG (Sucker ) TANAMAN NANAS (Ananas comosus (L .) Merr.) ASAL KAMPAR DENGAN PENAMBAHAN 6-BENZYLAMINOPURINE (BAP)

Abstract

Pineapple (Ananas comosus (L.) Merr.) is a fruit plant that is like d most people . Fruit production and seedling availability have to be balance d in order to provide consumer’s need . This problem can be solved using an in vitro technique . This research used Group Randomized Design ( GRD) Factorial with two factors which are concentration of BAP and explant section. The concentrations of BAP used in this study were 0,0; 0,5; 1,0; 1,5; 2,0; 2,5 mg/l on MS medium. Explant source was collected from sucker , with two different parts whole bud and bud sectioned longitudinally. The obje ctive s of this research were to get the optimal concentration, the best explant's cut part, and to get the best treatment interaction and combination for in vitro shoot induction. The r esult showed that the whole bud that could give response to BAP was indicated by shoot formation. The shoot was formed in the percentage of 25% with concentration 1,0 mg/l on 12 th day.