SINTESIS SENYAWA KALKON TURUNAN 3’,4’-DIMETOKSI ASETOFENON DAN UJI TOKSISITAS MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

Abstract

Three new chalcones of 3’,4’-dimethoxy acetophenone derivative were synthesized by stirrer method with alkaline sodium hydroxide catalyst at room temperature. They were (E) -1,3-bis (3,4 dimetoksifenil) prop-2-en-1-one (CA1), (E) -3 - (2,3 -dimetoksifenil) -1 - (3,4-dimetoksifenil) prop-2-en-1-one (CA2) and (E) -3 - (2,4,5-trimetoksifenil) -1 - (3,4-dimetoksifenil ) prop-2-en-1-one (CA3). Chalcone yield compounds obtained were 98%, 84.24% and 79.13%, respectively. The compounds were identified using UV spectroscopy, IR, 1H NMR and MS. Toxicity of chalcones tested using Brine Shrimp Lethality Test (BSLT) against larvae of Artemia salina Leach. CA1, CA2 and CA3 compounds had LC50 i.e., 17.022 mg / mL, 4.645 mg / mL and 648.634 mg / mL, respectively . The result showed that CA1 and CA2 were toxic as their LC50 values less than 200 mg / mL, while CA3 was found to be non- toxic compound.