PENGARUH pH DAN INOKULUM PADA PEMANFAATAN LIMBAH KULIT NENAS UNTUK PRODUKSI ENZIM SELULASE

Abstract

Utilization of waste is the best way to solve the problem of waste, in order to obtain the raw materials and to process waste into useful products which have higher added value. Pineapple waste per 100g wet weight contains of 86.7% water, 0.69% protein, 0.02% fat, 10.54% carbohydrate, 1.66% wet fiber and 0.48% ash. A large amount of carbohydrates in pineapple skin is very potential when it is used as a carbon source to produce cellulose enzymes. Cellulose enzyme is a complex of enzyme that can transform cellulose into glucose. To produce the Cellulose enzyme, fungus Aspergillus niger is used in solid fermentation processing. In this research, we have variations pH 4; 4.5, 5; 5.5 and 6, and the inoculum by 10%, 15%, 20%, 25% and 30% of the substrate, with 4 days fermentation. DNS method is used to test the glucose levels generated. The results obtained showed that the activity of the enzyme has the best obtained at 15% inoculum conditions and at pH 5 with the activity of the enzyme at 5.67 g / L. Enzyme with the best activity is used to hydrolyze the skin of pineapple into liquid sugar and from this process, liquid glucose concentrations obtained at 4.19 g / L