AKTIVITAS ANTIBAKTERI EKSTRAK DAUN BENALU (Scurulla sp) YANG TUMBUH PADA BEBERAPA INANG TERHADAP PERTUMBUHAN Salmonella typhi

Abstract

Scurulla sp as arboreal parasitic plant that hold and absorb its nutrient from host. During nutrient absorption, all of metabolites (include secondary metabolites) which produce by the host will be also absorbed. For this reason Scurulla sp must have capability to develop strategies to adapt. Different host are estimated to produce different secondary metabolite. The aims of this study is to know the best solvent between water and methanol to reduce the antibacterial activity of leaf extracts Scurulla sp from different hosts (Coffea arabica, Theobroma cacao, Diospyros nigra) and to know the concentration exctract of Scurulla sp against S. typhi. The method used in this research is the experimental method in the laboratory. There are 5 concentration variations. This extraction performed used methanol and water, and were used series of dilution 10%, 25%, 50%, 75%, and 100%. The exctract than was tested by paper disc diffusion and agar wells method, then incubated for 24-72 hours at room temperature. Observations were made by measuring the area which of inhibition. The results showed the inhibition of the solvent methanol has the inhibition area is bigger than the solvent water at all concentrations. Extract of Scurulla sp from Coffea arabica host, Theobroma cacao host and Diospyros nigra host showed the highest in a 100% concentration. The lowest concentration of 10% is characterized by no area of the inhibition on all hosts with the methanol and water solvents