FERMENTASI NIRA NIPAH (Nypa fruticans Wurmb) MENJADI BIOETANOL MENGGUNAKAN KHAMIR Pichia stipitis DALAM BIOFLO 2000 FERMENTOR

Abstract

World ethanol consumption for various use has increased significantly in recent years. Therefore it is necessary to have an alternative source of raw material for the production of bioethanol that can be improved. Nypa sap is one of the potential materials to be processed into bioethanol. Availability of land large enough nypa in Indonesia as well as a fairly high sugar content (15-20%) making nypa sap is a potential to be processed into bioethanol. Through the process of fermentation using yeast Pichia stipitis, glucose is converted into ethanol and carbon dioxide. Preparation of starter do with the yeast Pichia stipitis inoculum in the fermentation medium so that the yeast is able to adapt and ready for fermentation. Fermentation takes place in batches with a volume of 8 liters of fermentation medium, starter volume variation of 10%, 15%, 20%, and variations of fermentation time 6, 12, 24, 48, 72 and 96 hours. The degree of acidity of the fermentation medium is maintained at pH 4.5, stirring speed 200 rpm and temperature of fermentation at room temperature. Ethanol concentration was analyzed by using alcoholmeter. The fermentation process is shown in optimum condition starter volume increase of 20% and a fermentation time of 48 hours with initial sugar concentration of 150.999 mg / ml. The concentration of ethanol obtained in this condition is 9% (v / v) or 71.037 mg / ml with the acquisition of 92.244% yield.