ISOLASI DAN UJI TOKSISITAS EKSTRAK n-HEKSANA DAUN BENALU KARET (Helixanthera sp)

Abstract

Helixanthera sp is a species of parasite from the Loranthaceae family which can be used as a medicinal plant. This study aims to isolate and characterize secondary metabolites compounds from n-hexane extract of rubber parasite leaves (Helixanthera sp) and carry out a toxicity test using the Brine Shrimp Lethality Test (BSLT) method. Isolation was carried out using theb maceration method using methanol solvent and partitioned using n-hexane and ethyl acetate solvents. The crude n-hexane extract was separated using gravity column chromatography and produced 4 fractions. In fraction 1, dirty crystals were obtained in powder form and recrystallization was carried out to obtain pure compounds. The purity of the compound is determined by thin layer chromatography (TLC) test and melting point test. The pure compound obtained was 43,5 mg and was given the code HS-H-F1. The HS-H-F1 compound has a melting point of 158-160 ºC. Characterization of the HS-H-F1 compounds using UV-Vis spectroscopy showed maximum absorption at a wavelength of 278 nm, while the FTIR spectrum showed absorption at wave numbers 2946 cm-1 (aliphatic C-H), 1733 cm-1 (C=O), 1640 cm-1 (C=C) and 1248 cm-1 (C-O). The results of the toxicity test using the Brine Shrimp Lethality Test (BSLT) method showed that the n-hexane exctract of rubber parasite leaves (Helixanthera sp) was non-toxic with an LC50 value of ˃ 1000 ppm.