Abstract:
A research on the effects of lactic acid bacteria injection on the survival
rate of Epinephelus fuscoguttatus has been conducted from November until
December 2011 in the Laboratorium of BBPBAP Jepara. The aims of the research
was to understand the pathogenic effects of the lactic acid bacteria and to
understand non- specific defense as well as survival rate of E. fuscoguttatus that
were injected with the bacteria. Treatment applied was the different dosages of
bacteria injection. Each fish was injected with 0,1 ml of lactic acid bacteria with
10
5
cells/ml, 10
7
cells/ml and 10
9
cells/ml densities. The treated fish were reared
in 60 liter aquaria completed with aerators, 10 fish/ aquarium. The fish was feed
ad libitum with commercial fish feed pellets. The fish were reared for 14 days and
the clinical symptoms were checked daily. Before the injection and by the end of
the research, survival rate, body weight increment, total length increment,
leukocyte numbers, haematocrite and leucocrite levels were measured, while
water quality parameters (pH, salinity, DO, temperature and ammonia content)
were checked once/ 3 days.
The best result was obtained in the fish that was injected with 10
9
cells/ml
of lactic acid bacteria, as the survival rate was 100% and they showed highest
increment of body weight (from 13,20 g to 16,20 g), total length (from 8 cm to
9,77cm), leukocyte number (from 89,270 cells/ml to 128,610 cells/ml),
hematocrite level (from 22,3 % to 23,2%) and steady leucocrite level (1,0%).
Water quality parameters indicate that the quality of the water quality during the
research was good (DO 3,8 g/l – 4,3 g/l, temperature 26,2
0
C – 26,6
0
C, pH 7,6 –
7,8 , salinity 33 ppt and NH3 0,001 mg/l – 0,040 mg/l).
