ISOLASI DAN UJI ANTIOKSIDAN METABOLIT SEKUNDER DARI EKSTRAK ETIL ASETAT TANAMAN MIANA HIJAU-MERAH (Coleus hybridus)

Abstract

Miana (Coleus hybridus) is a species of the Lamiaceae family. This plant contains medicinal compounds that can be used as pain management, anti-inflammatory, antioxidant and antibacterial agents. Phytochemical tests showed that the green-red miana leaves contain flavonoid, terpenoid, steroid, saponin, phenolic and alkaloid. This study aimed to isolate secondary metabolites from green-red miana leaves and to test the antioxidants activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Secondary metabolite compounds from plants were isolated by maceration using methanol as solvent and partitioned using n-hexane and ethyl acetate as solvents. Vacuum liquid chromatography (VLC) and flash column chromatography were used to separated the compounds in the viscous ethyl acetate fraction. Brown solids were obtained in vial 38 as much as 17 mg which was coded as CHHM-EA-V6-FL38 and the thin layer chromatography test still showed 2 spots on the plate. The CHHM-EA-V6-FL38 subfraction was characterized by FT-IR spectroscopy. Based on the IC50 results of the CHHM-EA-V6-FL38 subfraction, it showed an IC50 value of 29.53 μg/mL at a concentration of 100 g/mL which indicated that this subfraction could be concluded to be active in the antioxidant test.