SELEKSI DAN UJI AKTIVITAS SELULASE ISOLAT BAKTERI ENDOFIT LBKURCC 364 s/d 391 DARI AKAR MANGROVE KABUPATEN BENGKALIS

Abstract

The Laboratory of Enzyme, Fermentation and Biomolecular Research, Faculty of Mathematics and Natural Sciences, Riau University has a collection of endophytic bacterial isolates from Mangrove roots in Sei Pakning Jetty and Tenggayun Beach, Bengkalis Regency, Riau. This isolate is thought to have the potential to produce an area of enzyme, therefore this study aimed to select 28 isolates of cellulase-producing bacteria using 1% CMC selective solid media, while for the production of cellulase enzymes in liquid media. Testing of cellulase enzyme activity was carried out using the 3,5-Di Nitro Salissilic Acid (DNS) method. The enzyme activity data obtained were then tested statistically using the Duncan's Multiple Range Test (DMNRT) method at a significance level of 5% and Principal Component Analysis (PCA). The results of the selection of 28 isolates of endophytic bacteria from the roots of the Bengkalis Regency on 1% CMC selective solid media obtained 26 isolates that were alive because they produced cellulase enzymes and 2 were dead. The highest cellulase activity of the 13 crude extracts tested was LBKURCC388 isolate (487±105) x 10-4 U/mL mangrove species Bruguiera sp. which is in cluster 1, and the lowest activity is isolate LBKURCC365 mangrove plant species Ceriops tagal (Perr.) C.B. Rob. with an activity value of (72±39) x 10-4 U/mL in the 4th cluster.